Preparation and characterization of peptide aggregations and their Au- labeled complex by transmission electron microscopy
نویسندگان
چکیده
The aim of this educational chapter is to provide a protocol describing how to prepare peptide fibrils and subsequently characterize them through Transmission Electron Microscopy (TEM) investigation, and functionalize them by metal nanoparticles labeling. The starting step is the monomer incubation and the final goal is the realization of a chemical bond between peptide material and metal nanoparticles.
منابع مشابه
Green Approach to Synthesis of Pt and Bimetallic Au@Pt Nanoparticles Using Carica Papaya Leaf Extract and Their Characterization
This study reports a green approach to synthesis of monometallic platinum nanoparticles (Pt NPs) and bimetallic aurium@platinum nanoparticles (Au@Pt) using aqueous leaf extract of Carica papaya as a reducing and stabilizing agent. The nature and morphology of as-synthesized PtNPs and bimetallic Au@Pt NPs were characterized using UV/vis spectroscopy (UV–vis), high resolution transmission electro...
متن کاملCobalt oxide nanoparticles by solid-state thermal decomposition: Synthesis and characterization
In this study, mononuclear octahedral cobalt(III) Schiff base complex [CoL3], L = (5-bromo-2-hydroxybenzyl-2-furylmethyl)imine, was synthesized from the reaction of Co(NO3)2•6H2O and the Schiff base ligand L in methanol as solvent. It used as a new precursor to prepare spinel type cobalt oxide nanoparticles by a facile solid-state thermal decomposition. Controlling the temperature and time, Co3...
متن کاملTransmission Electron Microscopy Sample Preparation of INCONEL 738 Nickel-Base Superalloy
Size, shape, volume fraction and distribution of embedded g/ phase in g phase has direct effect on strength of INCONEL alloy. Microstructure parameters of INCONEL phases are quantified from microstructure images using transmission electron microscopy (TEM). Different TEM sample preparation techniques were used to study INCONEL 738 alloy microstructure for transmission electron micros...
متن کاملOne-pot synthesis of Ag/Fe3O4 nanocomposite: Preparation, characterization and efficient catalyst
In this study, nanometer sized of Ag/Fe3O4 nanocomposite (AFN) have been synthesized by using a simple and based on chemical reduction method. The size and shape of (AFN) controlled by using of condensation (Fe2O3 (2.975 mmol), NaBH4 (21.25 mmol), AgNO3 (2.532 mmol) and polyvinylpyrrolidone (PVP) (8.925 mmol K30 Mr=10.000) in 40 ºC. The synthesized AFN was characterized by XRD, scanning electro...
متن کاملSynthesis of nanocrystalline BaTiO3 ceramics via hydrothermal condition and structural characterization by HRTEM and SAED
In the present work, we report a suitable approach for the preparation of BaTiO3 nanostructures via the hydrothermal condition using Dolapix ET85 as surfactant. The powders were investigated by X-ray diffraction (XRD), scanning election microscopy (SEM), energy-dispersive X-ray spectroscopy (EDAX), field emission transmission electron microscopy (FETEM), selected area electron diffra...
متن کامل